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. 2016 Nov 24;6:37727. doi: 10.1038/srep37727

Figure 5. Biochemical characterization of PKM2 and PKM1 isoforms on isolated photoreceptor cells from dark- and light-adapted Balb/c mice.

Figure 5

Retinal homogenates from dark- and light-adapted Balb/c mice were subjected to OptiPrep™ (8–40%) density gradient centrifugation (A). Fractions of inner segments and intact photoreceptors (B) were collected from the top to the bottom of the gradients. A ten-microliter sample (one-microliter for rhodopsin) was subjected to immunoblot analysis (C) with opsin, α-3 Na/K ATPase, pPKM2, PKM2, and PKM1 antibodies. Full-length blots are presented in the Supplementary Information.