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. 2016 Nov 24;6:37727. doi: 10.1038/srep37727

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Copyright © 2016, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Retinal homogenates from Nrl^−/− mice were subjected to OptiPrep™ (8–40%) density gradient centrifugation, and fractions of inner segments and intact photoreceptors (A) were collected from the top to the bottom of the gradients. A ten-microliter sample (three-microliter for M-opsin) was subjected to immunoblot analysis (B) with M-opsin, α-3 Na/K ATPase, pPKM2, PKM2, and PKM1 antibodies. Full-length blots are presented in the Supplementary Information.