(A) PCA plot of the 500 genes with the highest variances. (B) Venn diagram of the DEGs with a Padj value cutoff of 0.01 in different temperature comparisons52. (C)The heatmap showing the negative log10 Padj value of DEGs are shown for the six major clusters generated by the fold change pattern. The six major clusters are indicated by the grey coloured heatmap, which indicate the fold change direction for M/L and H/M, respectively. A fold-change value of <2 or Padj value >0.01 was considered as unchanged for this cluster generation. (D) The GO network of the top 15 enrichments of each cluster that satisfy a significant P < 0.05 of the hypergeometric test with FDR correction from BiNGO. The blue and red colours indicate enrichment of genes that are upregulated against high and low temperature, respectively. The GO terms that are upregulated in mid temperature are shown in green. ATPET, ATP synthesis coupled electron transport; C, chloroplast; CBP, cellular biosynthetic process; CelR, cellular respiration; CGMP, cellular glucan metabolic process; CMBP, cellular macromolecule biosynthetic process; CPMP, cellular polysaccharide metabolic process; CX, complex; Cy, cytosolic; ENV, envelope; ETC, electron transport chain; GMP, glucan metabolic process; INT, intracellular; LH, light harvesting; LRS, large ribosomal subunit; MBO, membrane-bound organelle; MCX, macromolecular complex; MP, metabolic process; ND, NADH dehydrogenase; NMBO, non-membrane-bound organelle; NMBO, non-membrane-bounded organelle; NRPLR, negative regulation of photosynthesis; OP, oxidative phosphorylation; OP, organelle part; OSC, organelle subcompartment; P, plastid; PHO, photosynthesis; PHOMEM, photosynthetic membrane; PMP, polysaccharide metabolic process; Q, quinone; R, ribosome; RETC, respiratory electron transport chain; RIB, ribonucleoprotein; RTAS, response to abiotic stimulus; RTLS, response to light stimulus; RTR, response to light radiation; SAMdma, S-adenosylmethionine-dependent methyltransferase activity; SCoR, structural constituent of ribosome; SMA, structural molecule activity; UQ, ubiquinone.