Figure 1. AMCase is not degraded by pepsin.

Soluble proteins obtained from mouse stomach were incubated at 37 °C for 0, 5, 10, 20, 40 and 60 min at pH 7.6 or 2.0. (a) Total protein analysis by Coomassie Brilliant Blue (CBB) staining, (b) Total protein levels quantification, (c) Western blot and (d) chitinolytic activity assay measured at pH 2.0 as described in Methods. Values in (b) and (d) represent mean ± SD from a single experiment conducted in triplicate. *p < 0.05. P-values were determined using Student’s t-test.