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. 2016 Nov 24;6:37756. doi: 10.1038/srep37756

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Copyright © 2016, The Author(s)

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(a) The mRNA levels of nine genes were quantified by qPCR. Upper panel, actual value; lower panel, logarithmic scale. Y axis represents molecules per 10 ng of total RNA. The numbers in the figure indicate relative expression levels with GAPDH set at 1.0. Pep C, pepsinogen C; Gif, gastric intrinsic factor; ATPase, H⁺/K⁺-ATPase. (b) Expression and co-localization of pepsin C and AMCase proteins in mouse stomach sections shown by immunohistochemistry using anti-pepsin C (green) and anti-human AMCase (red). Scale bars, 1,000 μm (upper panels); 50 μm (lower panels). (c) pH profile of pepsin and AMCase. Values in (a) and (c) represent mean ± SD. **p < 0.01. P-values were determined using Student’s t-test.