Figure 3. AMCase is resistant to digestion by trypsin and chymotrypsin.

Soluble proteins fraction from mouse stomach was incubated under stomach-like condition, followed by incubation in intestine-like environment containing trypsin and chymotrypsin at equal or 50-fold excess to the amount of the soluble proteins. The samples were analyzed by (a) CBB staining, (b,c) Western blot and (d) measurement of chitinolytic activity. All incubations were performed at 37 °C. Values represent mean ± SD. **p < 0.01 P-values were determined using Student’s t-test.