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. 2016 Nov 24;14:325. doi: 10.1186/s12967-016-1084-5

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© The Author(s) 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — In silico design of EBOV GP synthetic ORF. a Zaire EBOV Mayinga strain GP sequence and b deduced nucleotide sequence provided of a tag peptide (gD 106; highlighted in red) a Kozak’ s sequence (KS; highlighted in grey) and restriction enzyme sites (SmaI and NheI; highlighted in yellow) for subcloning