Fig. 4.

Kinetics of the humoral immune responses of goats immunized with BoHV-4-syEBOVgD106ΔTK. a Diagram showing the goats immunization scheme and blood sample collection. b Dilutes sera (1/10, 1/100, 1/1000 and 1/10,000) collected at 2 and 5 weeks (2 Ws; 5 Ws) from three (A, B and C) BoHV-4-syEBOVgD106ΔTK inoculated goats, were compared with pre-immune sera (PI) for anti-GP antibodies by ELISA. c Antibodies detected were expressed as the optical density at 450 nm and each value represents the mean response of the three goats sera, ± the standard error of the mean, at the same dilution and collected at the same time. Response differences between pre- and post-immune sera were measured by Student’s t test (*P ≤ 0.005)