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. 2016 May 13;7(25):37420–37435. doi: 10.18632/oncotarget.9357

Figure 7. Overexpression of miR-23a blocked rapamycin-induced anti-SIPS activity.

Figure 7

a. Prior to UV irradiation, cells were co-transfected with Ago-23a or control (Ago-CNT) and GFP-LC3 adenovirus. SA-ß-gal staining was performed. Scale bars = 100μm. b. The percentage of SA-ß-gal-positive cells were calculated (means ±SEM of the independent experiments, *p < 0.05, N.S., not significant). c. The cells were stained by EdU and Hoechst two days post UV irradiation. The percentage of EdU-positive cells was shown d. G1 arrest was examined. e. The relative protein levels of p53, p16, and p21 were examined. Actin was used as a loading control. The p53/Actin, p16/Actin and p21/Actin, densitometric ratios were marked (3 independent experiments gave similar results. See Figure S7). f. Prior to UV irradiation, Immunoblots of Ago-23a or control (Ago-CNT) transfected cells that were treated with DMSO or rapamycin(10 nM, 24h) (3 independent experiments gave similar results. See Figure S7). g. qRT-PCR analysis of miR-23a mRNA levels(means ±SEM of the independent experiments, **p < 0.01).