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. 2016 May 26;7(25):37471–37486. doi: 10.18632/oncotarget.9650

Figure 3. Identification of the acetylated site(s) of Osx mediated by CBP.

Figure 3

A. HEK 293T cells were transiently co-transfected with Flag-CBP-HA and HA-Osx-GFP expression plasmids or K26R, K41R, K45R, K46R, K58R, K230R, K291R, K307R, K312R mutants. The cell lysates were immunoprecipitated with an anti-HA antibody and then blotted with an anti-Acetylated-Lys antibody. Flag-CBP-HA and HA-Osx-GFP protein were detected by western blotting with an anti-HA antibody. B. HEK 293T cells were transiently transfected with HA-Osx-GFP expression plasmid or the K307R, K312R mutants. 24 h after transfection, the cells were treated with or without 60 μM TSA for 24 h. The cell lysates were immunoprecipitated with an anti-HA antibody and then blotted with an anti-Acetylated-Lys antibody. HA-Osx-GFP protein were detected by western blotting with an anti-HA antibody. Experiments were repeated at least three times.