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. 2016 Jan 27;7(25):37513–37523. doi: 10.18632/oncotarget.7038

Figure 6. Salmonella reduced vessel density and enhanced tumor cell apoptosis.

Figure 6

A. Tumors were excised at day 14, snap frozen and immunostained with rabbit antibody against factor VIII-related antigen (× 400). B. Tumors were excised at day 14, and a TUNEL assay was used to detect apoptotic cells (× 400). C. Intratumoral microvessel density was determined by averaging the number of vessels in three areas of highest vessel density at × 400 magnification in each section (n = 3, the data are the mean ± SD. *p < 0.05). D. TUNEL-positive cells were counted from three fields of high-density positive cells in each section to determine the percentage of apoptotic cells (n = 3, the data arethe mean ± SD. ***p < 0.001).