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. 2016 May 10;7(25):37693–37713. doi: 10.18632/oncotarget.9264

Figure 3. Ectopic expression of Survivin significantly attenuates entinostat potentiation of paclitaxel-induced apoptosis.

Figure 3

A549 and H460 cells infected with lentivirus containing either control vector pLEX-MCS (Control) or pLEX-hSurvivin (Survivin) were subjected to the following experiments. (A) Western blot analyses of Survivin or β-actin. (B and C) Cells were then treated with combinations of entinostat (3 μmol/L and 0.5 μmol/L for A549 and H460, respectively) and paclitaxel (6 nmol/L and 3 nmol/L for A549 and H460, respectively) for 24 h. Cells were collected and subjected to western blot analyses of PARP (F-PARP, full length PARP; C-PARP, cleaved PARP), caspase-8 (Pro-Casp-8, pro-caspase-8; C-Casp-8, cleaved caspase-8), caspase-3 (Pro-Casp-3, pro-caspase-3; C-Casp-3, cleaved caspase-3), or β-actin (B), or apoptotic-ELISA (C). Bars, S.D.