Table 4. Standard immune cell types in healthy donors following 48 hours treatment with epacadostat.
| Immune cell type | Epacadostat 0 | Epacadostat 0.25 μM | Epacadostat 1 μM | P-value |
|---|---|---|---|---|
| CD4+ | 38 | 38 | 38 | 0.85 |
| CD8+ | 27 | 27 | 26 | 0.56 |
| Tregs | 1.2 | 1.2 | 0.9 | 0.28 |
| B cells | 10 | 10 | 11 | 0.69 |
| NK | 1.7 | 1.6 | 1.6 | 0.08 |
| NKT | 0.7 | 0.7 | 0.7 | 0.43 |
| cDC | 0.16 | 0.17 | 0.16 | 0.37 |
| pDC | 0.004 | 0.005 | 0.004 | 0.77 |
| MDSC | 0.6 | 0.5 | 0.5 | 0.32 |
Human PBMCs from 10 healthy donors were subjected to multiparameter flow cytometric evaluation after 48 hours incubation with epacadostat (0, 0.25 or 1.0 μM). Nine standard immune cell types were analyzed. Values are displayed as the median frequency of PBMCs. There were no significant changes between the treatment groups (Wilcoxon matched-pairs signed rank test).
cDC, conventional dendritic cells; MDSC, myeloid derived suppressor cells; NK, natural killer; NKT, natural killer T cells; PBMCs, peripheral blood mononuclear cells; pDC, plasmacytoid DC; Tregs, regulatory T cells.