| primers used for genotyping of SNPs |
| rs753955-F |
5′-AATATAGGTGGGCCCTGTCC-3′ |
| rs753955-R |
5′-GGGAAAGACAATGCTGTGGT-3′ |
| rs2853677-F |
5′-CCAATCCAGTCTGACAGTCG-3′ |
| rs2853677-R |
5′-GAAACAAGGGAACGAGGACA-3′ |
| rs2736100-F |
5′-GTGCTGTTTTCCCTGCTGAC-3′ |
| rs2736100-R |
5′-GGGAACAAAGGAGGAAAAGC-3′ |
| rs4488809-F |
5′-ATGCAAGCATCTGCTCTTGA-3′ |
| rs4488809-R |
5′-TGTGCATTCCTGTGTTTCCT-3′ |
| rs2741354-F |
5′-GGCCAACACAAGGACTGACT-3′ |
| rs2741354-R |
5′-ATTTCGCTGCAGCTTCTTTC-3′ |
| rs12296850-F |
5′-AGGATTCATGGGATCAGTGG-3′ |
| rs12296850-R |
5′-GTAGGTCCCACAGGGAGTGA-3′ |
| rs753955-SBE |
5′- (GACT)1ATCATGTGAAGGCTTGAA-3′ |
| rs2853677-SBE |
5′- (GACT)3TTTGTCACTAGAGACCCG-3′ |
| rs2736100-SBE |
5′- (GACT)5TCCGTGTTGAGTGTTTCT-3′ |
| rs4488809-SBE |
5′- (GACT)7TGCTCTTGAGGCAGTAAA-3′ |
| rs2741354-SBE |
5′- (GACT)9GGTATCACCCTAAACCAAG-3′ |
| rs12296850-SBE |
5′- (GACT)10CACATATAAGTAAAAGGGCTTAC-3′ |
| primers used for cloning |
| SNAI1-CDS-F-Nflag |
5′-CGGGATCCGCCACCATGGACTACAAGGACGACG ATGACAAGCCGCGCTCTTTCCTCGTCAGGA-3′ |
| SNAI1-CDS-R |
5′-CGGGATCCTCAGCGGGGACATCCTGAGCAGCCG GACTCTTGGT-3′ |
| primers used for luciferase assay |
| TERT-A |
5′-AGGAAGCTTCGCTGCCTGAAACTCGC-3 |
| TERT-S500 |
5′-TCACTCGAGGCATTCGTGGTGCCCG-3′ |
| TERT-S1000 |
5′-GTCCTCGAGTGTCCTGCGGTTGTGCC-3′ |
| SNP-F |
5′-CGGGGTACCATGTGAGGCATTGTTAGGTGCAT-3′ |
| SNP-R |
5′-CGGGAGCTCCTAAGTCAGCAGGGAAAACAGCA-3′ |
| primers used for EMSA |
| Snail-EMSA-F1-biotin |
5′-ACTAGAGACCCGCCTGGTGCACTCTG-biotin |
| Snail-EMSA-R1-biotin |
5′-CAGAGTGCACCAGGCGGGTCTCTAGT-biotin |
| Snail-EMSA-F2-biotin |
5′-ACTAGAGACCCGTCTGGTGCACTCTG-biotin |
| Snail-EMSA-R2-biotin |
5′-CAGAGTGCACCAGACGGGTCTCTAGT-biotin |
| primers used for ChIP |
| ChIP-S |
5′-ACTCTTGGACTCAAGGGATC-3′ |
| ChIP-A |
5′-ACACTCGGCAGGAAACG-3′ |
| E-Cad-S |
5′-CAACTCCAGGCTAGAGGGTCACCGC-3′ |
| E-Cad-A |
5′-AACTGACTTCCGCAAGCTCACAGG-3′ |
| β-actin-S |
5′-GCCAACCGCGAGAAGATGACCCAGA-3′ |
| β-actin-A |
5′-GAGTCCATCACGATGCCAGTAG-3′ |
| primers used for RT-PCR |
| TERT-Q-F |
5′-CCGATTGTGAACATGGACTACG-3′ |
| TERT-Q-R |
5′-CACGCTGAACAGTGCCTTC-3′ |
| SNAI1-Q-F |
5′-ACTGCAACAAGGAATACCTCAG-3′ |
| SNAI1-Q-R |
5′-GCACTGGTACTTCTTGACATCTG-3′ |
| GAPDH-F |
5′-GTCAACGGATTTGGTCGTATT-3′ |
| GAPDH-R |
5′-AGTCTTCTGGGTGGCAGTGAT-3′ |
| primers used for 3C |
| 3C-1 |
5′-CCAGCCCCTCCCCTTCCTTT-3′ |
| 3C-2 |
5′-GCTCCAGGCACAACGAACGC-3′ |
| 3C-3 |
5′-GGAAGATGAGCGTGCGGGACT-3′ |
| 3C-4 |
5′-CGGTGGCTCACGCCGGTAAT-3′ |
| 3C-5 |
5′-CCCCAGGTGTCCTTGGCGTTTG-3′ |
| 3C-6 |
5′-GTTTGGTAGCATTTATGTGAGGCATTG-3′ |
| 3C-7 |
5′-TGTGCGGTGTCTGGATGG-3′ |
| control 1-S |
5′-GGGGAACCAGCGACATGC-3′ |
| control 1-A |
5′-CCCTGAACACCCACAAACACT-3′ |
| control 2-S |
5′-GCTTGCTCCTGAATGTTTGCT-3′ |
| control 2-A |
5′-TGGTGAGAAACAAGGGAACGAG-3′ |
| control 3-A |
5′-CTCCATACATCCAGCTCACC-3′ |
| control 3-S |
5′-TGGGAACCAGGACAAAGG-3′ |
