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. 2016 May 13;7(25):37868–37881. doi: 10.18632/oncotarget.9350

Figure 4. The inhibition of TIMP2/3 by DANCR depends on EZH2.

Figure 4

(A) DZNep, an inhibitor of EZH2, decreases EZH2 protein level in both C4-2B and CW22Rv1 cells, as detected by western blot analysis. (B) Both DANCR knockdown and DZNep treatment increases the expression of TIMP2/3 mRNA in C4-2B and CW22Rv1 cells as detected by RT-qPCR assay. (C, D) Both DANCR knockdown and DZNep treatment increases the expression of TIMP2/3 protein in C4-2B and CW22Rv1 cells as detected by western blot analysis. (E, F) Both DANCR knockdown and EZH2 inhibition decreases invasion and migration of C4-2B and CW22Rv1 cells as detected by transwell assay. (G, H) Both DANCR knockdown and EZH2 inhibition decreases the migration of C4-2B and CW22Rv1 cells as detected by wound healing assay. Each experiment was repeated at least three times and a representative experiment is shown. *p < 0.05, **p < 0.01, ***p < 0.001.