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. 2016 May 13;7(25):37868–37881. doi: 10.18632/oncotarget.9350

Figure 6. AR inhibits the expression of DANCR, up-regulates TIMP2/3 and suppresses invasion and migration of prostate cancer cells.

Figure 6

(A) Androgen inhibits the expression of DANCR in C4-2B and CW22RV1 cells, as detected by RT-qPCR assay. The mRNA level of DANCR were assayed after 24 hours of additional incubation with 1 nmol/L or 5 nmol/L DHT. (B) Knockdown of AR increases the expression of DANCR in C4-2B and CW22RV1 cells, as detected by RT-qPCR assay, and expression of AR was measured by western bot analysis. (C, D)Androgen treatment increases, while AR knockdown decreases the expression of TIMP2/3 protein in C4-2B and CW22RV1 cells as detected by western blot analysis. (E, F) Androgen treatment increases the expression of TIMP2/3 mRNA in C4-2B and CW22RV1 cells as detected by RT-qPCR assay. (G, H) AR knockdown decreases the expression of TIMP2/3 mRNA in C4-2B and CW22RV1 cells as detected by RT-qPCR assay. (I, J) Knockdown of AR increases the invasion and migration of C4-2B and CW22RV1 cells as detected by transwell assay. *p < 0.05, **p < 0.01, ***p < 0.001.