Figure 2. CAPE treatment affected abundance and phosphorylation status of proteins regulating cell proliferation and tumor metastasis.

Expression level and phosphorylation status of signaling proteins involved in cell cycle regulation, Wnt signaling, PI3K-Akt signaling, and NF-κB in PC-3 and DU-145 PCa cells treated with 0, 20, 40, 80 μM CAPE for 24 h was determined by Micro-Western Array with 264 different antibodies. A representative image of PC-3 and DU-145 PCa cells being assayed with Micro-Western Array was shown, respectively. Proteins with expression level increased or decreased at least 1.5 fold under CAPE treatment in PC-3 (C) and DU-145 (D) cells were demonstrated by heatmap.