Figure 3. CAPE treatment suppressed expression of signaling proteins involved in canonical Wnt signaling and EMT but induced expression of signaling proteins in non-canonical Wnt signaling.

Heatmap illustration and Western blotting images of the expression levels and phosphorylation status of signaling proteins regulating canonical Wnt Signaling, non-canonical Wnt signaling and EMT in PC-3 cells (A–B) and DU-145 (C–D) treated with different concentrations of CAPE for 24 h were determined by conventional Western blotting assay. Expression of β-actin was used as loading control for whole cell lysate. Expression of Lamin A/C and GAPDH was used as loading control for nuclear and cytoplasmic protein extract, respectively, in nuclear-cytoplasmic separation lysate.