Figure 4. CAPE treatment suppressed migration of PC-3 and DU-145 PCa cells via induction of ROR2 as well as suppression of EMT and canonical Wnt signaling.

(A) Migration of DU-145 and PC-3 PCa cells transfected with shROR2 or control vector were treated with or without 20 μM CAPE for 24 h. Migration ability of cells were determined by transwell assay. (B) Proteins levels of ROR2, E-cadherin and vimentin in DU-145 and PC-3 PCa cells with or without shROR2 knockdown in the presence or absence of 20 μM were determined by Western blotting assay. Expression of β-actin was used as loading control. (C) DU-145 and PC-3 cells were co-transfected with vector containing eight copies of a TCF-LEF response element driving the transcription of the luciferase reporter gene luc2P and phRL-CMV-Renilla luciferase vector. Cells were treated with 0, 20, 40, or 80 μM CAPE for 24 h. Intensity of luciferase was measured with Dual-Luciferase kit (Promega) and a Monolight luminometer to determine the canonical Wnt signaling. Asterisks *, **, and *** represent statistically significant difference p < 0.05, p < 0.01, and p < 0.001, respectively, between the groups being compared.