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. 2016 May 19;7(25):38133–38142. doi: 10.18632/oncotarget.9477

Figure 1. Cell morphology and density regulate S100A7 induction and YAP activity, and subcellular location of S100A7 and YAP are detected in dense cells.

Figure 1

(A) Western blot analyses of S100A7, YAP and pYAP (S127) in the indicated cells. Cells were cultured in suspension for two days (S48 h) and then reattachment for one day (S48 h-reattached). Cells were cultured densely for two days (D48 h) and then relief from dense culture (D48 h-sparse). GAPDH was used as a loading control. (B) The expression of S100A7 and CTGF or CYR61, two endogenous markers of YAP, were analyzed by qPCR. Error bar, SD of three different experiments.*p < 0.05, **p < 0.01; t-test. (C) Dense culture induced S100A7 expression and caused YAP nuclear exclusion in A431 cells. Cells were cultured in dense for two days. Samples were then stained with anti-S100A7 (Abcam) and anti-YAP (CST) antibodies. DAPI is a nuclear counterstain. Scale bar, 20 μm.