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. 2016 May 19;7(25):38133–38142. doi: 10.18632/oncotarget.9477

Figure 3. S100A7 induction is regulated by activation of the Hippo pathway in A431 cells, and TEAD1 mediates YAP-dependent S100A7 expression.

Figure 3

(A) Depletion of YAP using siRNA in normal attached A431 cells. The expression of YAP, S100A7 and pYAP (S127) were determined by Western blotting. β-actin was used as a loading control. (B) qPCR analyses of S100A7, YAP, CTGF or CYR61 in the indicated cells after silencing of YAP. Error bar, SD of three different experiments. *p < 0.05, **p < 0.01; t-test. (C) Overexpression of LATS1 in normal attached A431, cells. Anti-Flag tag antibody was used to judge the transfection efficiency. (D) Overexpression of YAP-WT and mutant activated YAP-S94A in normal attached A431 cells. Anti-Flag tag antibody was used to judge the transfection efficiency. (E) The mRNA expression of CYR61, and S100A7 were examined using qPCR. Error bar, SD of three different experiments. *p < 0.05, **p < 0.01; t-test. (F) Western blot analyses for S100A7 expression after TEAD1 silencing in normal attached A431 cells, with β-actin as a loading control.