Figure 2. Short-term (3 h) treatment with both inhibitors does not upregulate the expression of Hsp90/Hsp70 but enhances the radiosensitizing effect of NVP-AUY922 in tumor cells.

(A) Western blot analysis of the expression levels of Hsp90 and Hsp70 proteins in DMSO-treated controls and drug-treated (3 h) GaMG, SNB19, SW480 and SW48 cells. (B) Clonogenic survival of irradiated tumor cell lines pre-treated for 3 h with PI-103, NVP-AUY922, or both. The cells were plated for the colony-forming test 24 h post-IR. (Take note of the positions of curves 3 and 4). (C) Mutational status of tested cell lines with respect to p53 and PTEN as well as the radiosensitizing effect of PI-103. For further details, see Legend to Figure 1.