Figure 2. CD46 expression analysis and Ad5/35-mediated gene transduction in colorectal cancer cell lines.

A. CD46 expression in colon cancer cell lines was analyzed by Western blot analysis. B. CD46-positive cells were detected by flow cytometry in response to different dose of Ad5/35-GFP. C-E. Colon cancer cells were applied to flow cytometry to demonstrate intensity of CD46 expression (C) or Ad5/35-mediated reporter transduction (D). Percentage of cells double-stained with CD46 and GFP was shown in E. Error bars represent SEM. Statistics: B, p<0.01 by 1-way ANOVA and post-hoc Tukey test; C-D, p=0.267 by 2-way ANOVA.