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. 2016 May 20;7(25):38319–38332. doi: 10.18632/oncotarget.9513

Figure 5. Acetylation-mimic ERG increases prostatic cell invasion.

Figure 5

(A) Western blot for ERG and p300 with over-expressed HA-ERG full-length wild-type (WT) or HA-ERG full-length acetylation-mimic (KQ), and p300 siRNA knockdown in BPH-1 cells. As controls, cells were transfected with non-targeting siRNA (siNT), and empty vector (EV). ERK2 as a loading control. p300 protein levels were normalized to ERK2 first and then to the value for cells transfected with EV and siNT. (B) Number of BPH-1 cells that invaded through basement membrane and matrix with over-expressed empty vector, HA-ERG wild-type, HA-ERG acetylation-mimic (KQ), with or without p300 siRNA. *P < 0.05, **P < 0.01, ***P < 0.001. (C) Representative images from BPH-1 cell invasion assay. Scale bar, 200 μm.