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. 2016 May 20;7(25):38333–38346. doi: 10.18632/oncotarget.9518

Figure 4. Effects of EZH2 silencing on the expression of metastasis-associated proteins and stem cell markers in osteosarcoma cell lines.

Figure 4

(A) The levels of MT1-MMP, MMP-2, and MMP-7 were detected in cells after transfection with non-specific siRNA or EZH2 siRNA and determined by western blot analysis. (B) Western blots from A were analyzed by densitometry, which was carried out using Image J software and normalized to β-actin expression. (C) The levels of CD44 and Notch-3 were detected in cells after transfection with non-specific siRNA or EZH2 siRNA and evaluated by western blot analysis. (D) Western blots from C were analyzed by densitometry as described in Materials and Methods. All the results were reproducible in three independent experiments. The data are presented as the mean ± SD with **P < 0.001.