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. 2016 May 20;7(25):38380–38391. doi: 10.18632/oncotarget.9526

Figure 5. MLN4924 enhanced IR-induced apoptosis and DNA damage.

Figure 5

(A and C) MLN4924-IR induced more apoptotic cells than IR alone. Subconfluent cells were treated with MLN4924 (DU145 at 200 nM and PC3 at 150 nM) or IR (4 Gy) or MLN4924+IR, 72 hours later, one portion of cells was subjected to Annexin V-FITC/PI double-staining analysis for apoptosis (A); the other portion was for IB analysis (C). (B) MLN4924 enhanced DNA damage in IR-treated cells. DU145 and PC3 cells were treated with MLN4924 (DU145 at 200 nM and PC3 at 150 nM) or IR (4 Gy) or MLN4924+IR for 24 hours, followed by IB analysis, using antibodies against p-H2Ax, t-H2Ax, CDT1, and ORC1, with GAPDH as a loading control. Shown is mean ± SEM, (n = 3): *P < 0.05, **P < 0.01, ***P < 0.0001. MLN, MLN4924; IR, irradiation.