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A. PC cells were seeded into the upper chambers of Transwell plates under serum-free conditions and cultured for 8 h. B. PC cells were seeded into Matrigel-coated upper chambers of Transwell plates and cultured for 24 h. The number of stained cells was counted in 10 randomly selected fields of view with a microscope at 200× magnification. * P < 0.05, ** P < 0.01, and *** P < 0.001.
