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. 2016 May 23;7(25):38598–38611. doi: 10.18632/oncotarget.9560

Figure 2. Roscovitine treatment activates p53 and induces p53-dependent suppression of HPV-positive cells growth.

Figure 2

A. HPV+ UMSCC47 cells were transiently transfected with control or p53 shRNA and plated for survival after the treatment with increasing doses of roscovitine; standard deviations are calculated from two independent experiments. B. Relative mRNA levels of p53 target gene CDKN1A (p21) in HPV-positive SCC090 cells treated or not with 20 μM roscovitine for 24 hours; standard deviations are calculated from two independent experiments. C. SCC090 cells were treated or not with roscovitine, lysed, and immunoblotted with indicated antibodies. D. HPV-positive UMSCC47 and SCC090 cells were treated or not with roscovitine; the cells were collected and HPV16 mRNA levels were determined in qRT-PCR (top), or p53 protein levels were determined in Western Blot (bottom). E. HPV-positive SCC090 cells were treated for 24 hours with roscovitine, or zeocin, or left untreated as a control, lysed and immunoblotted with indicated antibodies.