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. 2016 May 24;7(25):38638–38657. doi: 10.18632/oncotarget.9575

Figure 5.

Figure 5

A. Expression of Sox2 mRNA in GBM1- and GBM2-PrP-KO cells, evaluated by quantitative RT-PCR. Sox2 expression values were normalized to respective GBM-Scr cells (white bars). Results are expressed as means ± SEM. **p<0.01 vs. respective GBM-Scr cells. B. Representative immunoblots of Sox2 protein expression level in GBM-Scr and GBM PrP-KO cells. Immunoblot analysis of β-actin levels was performed to normalize the data for the total protein input of cell lysates. C. Densitometric analysis of Sox2 expression from three independent Western blot experiments and expressed as percentage of GBM-Scr cells ± SEM. **p<0.01 vs. respective GBM-Scr cells.