Figure 9. Effect of PrP^C down-regulation on the ability of GBM CSCs to form endothelial tubes.

A. Representative micrographs of tube formation in GBM-Scr and GBM-PrP-KO cells after 24h of growth in endothelial differentiation medium. B. Mean loops tube perimeter and C. mean branching points formation, induced in GBM cells by endothelial transdifferentiation. Perimeters are expressed in pixel. Data are expressed as percentage of respective GBM-Scr cell value. Each point represents the average of three independent experiments. **p<0.01 vs. GBM1-Scr cells, °°p<0.01 vs. GBM2-Scr cells