Figure 5. The expression of BCL-W does not change during mitotic block but varies widely among different cell lines.

A. BCL-W does not vary during mitotic block. HeLa cells were synchronized in G₂ (using a double thymidine procedure) or further blocked in mitosis with NOC for 4 h before harvested at the indicated time points. Lysates were prepared and analyzed with immunoblotting. The accumulation of cyclin B1 and destruction of cyclin A2 confirm the cells were trapped in mitosis. Mitotic cell death was corresponded with an increase in cleaved PARP1. The asterisks indicate the phosphorylated forms of BCL-2 and BCL-XL. B. BCL-W expression varies widely among different cell lines. The expression of BCL-2, BCL-XL, BCL-W, and MCL-1 was detected in different cell lines. Both normal or non-transformed immortalized cells (MCF10A, MIHA, IMR90, NP460, NP331, and NP550) and cancer cell lines (the rest) were compared.