Figure 6. Effects of Paf15 expression on cell viability & cell cycle after radiation.
RC cells with varying Paf15 expression levels were treated with gamma irradiation or plated without radiation as a control, then subjected to CCK-8 assay & flow cytometry at 0, 24 and 48 hours. A. & B. Growth rate, expressed as CCK-8 value percentages of unirradiated controls, showed a significant difference in cell proliferation at 48 h after radiation (P<0.05). C. Decreased Paf15 expression alleviated radiation-induced G2/M phase arrest in SW1463 at 24 hours after radiation (P<0.05) and D. in SW873 at both 24 and 48 hours after radiation (P<0.05). Vertical axis represents G2/M phase cell percentages of unirradiated controls.