Figure 5.

The enhanced cellular immune responses in peptide-immune C57BL/6 and the timeline of immunization. (A) Scheme of peptide immunization. Mice were primed and boosted with GP6 and NP1 packaged with Freud's adjuvants (Pep Immun group) or PBS (Mock group). Ten days after the final boost, splenocytes from mice were harvested for subsequent assays. (B) IFN-γ ELISPOT analysis of splenocytes obtained from HTNV peptide-immunized mice. Cells were stimulated with purified HTNV NP. (C) Splenocytes from immunized mice, restimulated with relative peptide were used as effector cells. As targets, EL-4 cells were pulsed with GP6 or NP1 at a concentration of 10 μg/ml. (■) represents NP1 immunization; (•) represents GP6 immunization; (▴) represents a positive control from the HTNV-challenged mice; (□) represents vaccine control; (◦) represents PBS control; (▾) represents adjuvant control. Data are expressed as the mean ± SEM (n = 6). ^***P < 0.0001.