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. 2016 Nov 25;7:1785. doi: 10.3389/fpls.2016.01785

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Copyright © 2016 Guo, Shi, Yang, Yan, Zhong, Liu, Xia and Li.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effects of alkali stress on the contents of pigment chlorophyll a (A), chlorophyll b (B), chlorophyll a+b (C) and carotenoid (D) in young and old leaves of cotton seedlings. The values are the means ± SE of five biological replicates, and each replicate consisted of a pool of five plants. Significant difference between old and young leaves under similar stress condition was determined by t-test and marked as ^∗(P < 0.05) and ^∗∗(P < 0.01). The seedlings were subjected to 80 mM alkali stress (NaHCO₃:Na₂CO₃ = 9:1; pH = 9.06) for 6 days.