Table 3.

Signal peptide, N-glycosylation, and subcellular location prediction for the subtilases putatively involved in grapevine immunity.

Nucleotide	Sequence identification (NCBI)	VvSBT group	Chromosomal location	selection	Signal peptide prediction, signalP 4.1 (position)	N-glycosylation prediction (Asn-X-Ser/Thr), NetNGlyc 1.0 Server (position and sequence)	Subcellular location prediction
XM_002273159.3	Subtilisin-like protease SBT3.5	VvSBT3	16	Similarity to AtSBT3.3	YES (1–27)	YES	Extracellular region
						(184 NAT)
						(212 NTT)
						(373 NRT)
						(385 NHT)
						(412 NDT)
						(646 NNS)
						(682 NST)
						(689 NVT)
						(697 NST)
XM_002275345.2	Subtilisin-like protease SDD1	VvSBT5	2	Similarity to P69C	NO	YES *	Apoplast; Plant-type cell wall
						(15 NGT)
						(259 NCS)
						(314 NVS)
						(951 NDT)
XM_002275374.2	Subtilisin-like protease SDD1	VvSBT5	2	Similarity to P69C	YES (1–31)	YES	Apoplast; Plant-type cell wall
						(137 NRS)
						(186 NGT)
						(366 NFS)
						(382 NQT)
						(526 NVT)
						(644 NCS)
XM_002275393.2	Subtilisin-like protease	VvSBT5	2	Similarity to P69C	YES (1–20)	YES	Apoplast; Plant-type cell wall
						(306 NST)
						(510 NRT)
						(615 NDT)
XM_002275435.2	Uncharacterized protein LOC100242816	VvSBT5	2	Similarity to P69C	YES (1–31)	YES	Apoplast; Plant-type cell wall
						(254 NGT)
						(338 NGS)
						(398 NAS)
						(542 NDT)
						(656 NRT)
						(699 NSS)
						(1053 NTT)
						(1196 NST)
XM_002277863.3	Subtilisin-like protease	VvSBT5	7	Rpv9	YES (1–23)	YES	Apoplast; Plant-type cell wall
						(184 NFT)
						(216 NSS)
						(247 NGT)
						(298 NNS)
						(421 NAT)
						(460 NKS)
						(588 NDT)
						(614 NAT)
						(687 NYT)
						(719 NLT)
XM_002278414.3	Subtilisin-like protease SBT3.3	VvSBT3	2	Similarity to AtSBT3.3	YES (1–35)	YES	Extracellular region
						(192 NST)
						(219 NIT)
						(424 NAT)
						(642 NIS)
						(734 NLT)
XM_002284065.3	Subtilisin-like protease	VvSBT5	7	Rpv9	YES (1–24)	YES	Extracellular region
						(172 NFT)
						(378 NAS)
						(633 NYS)
XM_003634104.2	Subtilisin-like protease SBT3.5	VvSBT3	16	Similarity to AtSBT3.3	YES (1–27)	YES
						(184 NAT)
						(212 NTT)
						(373 NRT)
						(385 NHT)
						(412 NDT)
						(646 NNS)
						(682 NST)
						(689 NVT)
						(697 NST)
XM_003634105.2	Subtilisin-like protease SBT3.5	VvSBT3	16	Similarity to AtSBT3.3	YES (1–27)	YES	Extracellular region
						(184 NAT)
						(212 NTT)
						(373 NRT)
						(385 NLT)
						(412 NDT)
						(646 NNS)
						(682 NST)
						(689 NVT)
						(697 NST)
						(722 NST)
XM_010649370.1	Subtilisin-like protease SBT3.5	VvSBT3	3	Similarity to AtSBT3.3	YES (1–27)	YES	Extracellular region
						(179 NRS)
						(362 NQT)
						(407 NAT)
						(651 NTT)
XM_010659200.1	Subtilisin-like protease SBT5.3 isoform X2	VvSBT4	12	Located within Rpv1 + Rpv13	NO	YES *
						(60 NSS)
						(95 NGT)
						(155 NDS)
						(245 NAS)
						(318 NFT)
						(330 NST)
						(455 NAS)
						(500 NQT)
XM_010660203.1	Cucumisin isoform X1	VvSBT1	13	(Figueiredo et al., 2008, 2012; Monteiro et al., 2013)	YES (1–29)	YES	Extracellular region
						(180 NFT)
						(387 NRS)
						(460 NST)
						(640 NGT)
						(666 NRT)
XM_010663620.1	Subtilisin-like protease SBT3.5	VvSBT3	2	Similarity to AtSBT3.3	YES (1–36)	YES	Extracellular region
						(188 NST)
						(216 NTS)
						(247 NVS)
						(377 NKT)
						(925 NTT)
						(956 NAS)
						(1086 NVT)

Asterisk (

^*) indicates sequences that may not contain a signal peptide.