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. 2016 Nov 25;7:1761. doi: 10.3389/fpls.2016.01761

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Copyright © 2016 Wang, Jia, Huh, Muchlinski, Peters and Tholl.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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TPS20c from Cvi functions as a dolabellane type diterpene synthase. (A) GC-MS chromatogram of a culture extract from Escherichia coli coexpressing TPS20c and GGPP synthase. Ev+GGPPs, extract of E. coli culture expressing GGPP synthase only. Numbers indicate TPS20c products that were found in vitro and in planta. Arrows indicate other minor TPS20c diterpene products (B,C) Mass spectra for product 7 (dolathaliatriene) and product 8 (dolabelladienol). Mass spectra of the other numbered products are listed in Supplementary Materials (Supplementary Figure S4). (D) Chemical structures for dolathaliatriene and dolabelladienol.