FIGURE 7.

TPS9 from the Col ecotype functions as a diterpene synthase. (A) GC-MS chromatogram of a culture extract from E. coli coexpressing TPS9 and GGPP synthase. (B) GC-MS detection of diterpenes produced by TPS9 in the Col root. Diterpenes extracted from root tissue were compared with TPS9 diterpene products made from recombinant expression as labeled with lines. (C) Mass spectrum of the major product 1. Mass spectra of minor TPS9 diterpene products (marked with arrows in A) are listed in Supplementary Materials (Supplementary Figure S13).