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. 2016 Nov 25;7:1761. doi: 10.3389/fpls.2016.01761

FIGURE 8.

FIGURE 8

Analysis of products formed by recombinant TPS22 and TPS25 enzymes from (E,E)-FPP using SPME-GC-MS. TPS22 and TPS25 proteins without the N-terminal transit peptide were used for enzyme activity assays. (A,B) GC-MS chromatograms of enzymatic products formed by recombinant TPS22 and TPS25. Sesquiterpene products were identified by comparison with authentic standards (1 and 7) or by library suggestion (for 2, 3, 4, 5, and 6). 1, (E)-β-farnesene; 2 and 3, α-farnesene isomers; 4, β-bisabolene; 5 and 6, bisabolene isomers; 7, (E)-nerolidol; B: background. (C) GC-MS analysis of products formed by extracts of E. coli expressing the pET102 vector without an insert.