Table 3.
Effect of Se supplementation on the studied biological parameters, including markers of selenium status, oxidative stress and DNA damage. Data presented regardless of genotype
| Marker | Median and range, measured at four different time points | p value (MANCOVA)** | |||
|---|---|---|---|---|---|
| Baseline | 2 weeks | 6 weeks | Washout | ||
| Se (µg/L) | 62.65a
(34.32–109.12) |
98.00
b
(56.49–183.86) |
93.84
b
(54.92–148.76) |
74.61
c
(49.27–111.58) |
p < 0.0001 |
| Sepp1 * (ng/mL) | 3.86a
(0.86–36.03) |
7.36
b
(0.97–41.60) |
6.14
c
(0.26–34.08) |
6.00
c
(0.66–31.20) |
p < 0.0001 |
| GPx1 (U/gHb) | 17.10a
(9.68–29.04) |
16.24a
(8.93–28.17) |
24.43
b
(11.95–29.88) |
23.76
b
(14.03–29.96) |
p < 0.0001 |
| GPx3 (U/mL) | 0.18a
(0.12–0.25) |
0.18a
(0.12–0.26) |
0.20
b
(0.14–0.28) |
0.19
c
(0.12–0.26) |
p < 0.0001 |
| SOD1 (U/mg Hb) | 6.20 (3.93–8.39) |
6.12 (3.04–9.38) |
6.02 (4.34–8.50) |
6.00 (3.93–8.75) |
p = 0.21 |
| Cp (g/L) | 0.46a
(0.24–0.91) |
0.49
b
(0.30–0.87) |
0.48
b
(0.34–0.79) |
0.49
b
(0.34–0.78) |
p = 0.01 |
| TAC * (mmol/L) | 0.85a
(0.03–5.08) |
1.18
b
(0.10–4.99) |
1.36
c
(0.05–5.25) |
2.02
d
(0.24–5.95) |
p < 0.0001 |
| TBARS * (mmol/mL) | 1.75a
(1.00–4.08) |
1.98
b
(1.02–4.24) |
2.02
b
(1.02–4.52) |
2.08
b
(1.01–3.29) |
p < 0.0001 |
| RFT (MFI index) | 78.90a
(22.35 ± 134.12) |
67.66
b
(32.18 ± 113.57) |
na | na | p < 0.0001 |
| DNA strand breaks (% tail DNA)* | 1.86a
(0.57–5.50) |
na | 1.91a
(0.63–3.90) |
2.10
b
(1.02–6.08) |
p < 0.0001 |
| DNA oxidation (% tail DNA) | 2.58a
(0.00–5.74) |
na | 2.35a,b
(0.00–6.23) |
3.08
c
(0.00–6.49) |
p < 0.0001 |
Statistically significant p values (last column) and values significantly different as compared to baseline are typed in bold. Values indexed with different upper case letters (a, b, c, d) are significantly different at p ≤ 0.05 (contrast test for different time points, adjusted for age, sex, BMI, and baseline plasma Se)
na parameter was not analyzed at this time point
* Skewed data; for these parameters, p values were calculated for log-transformed data
** Model adjusted for age, sex, BMI, and baseline plasma Se