Fig. 4. Overexpression of SLC6A12 enhanced migration and invasiveness of SK-OV-3 cells. Migration of serum-starved cells towards 15% serum-containing medium was determined by transwell assay. Representative images of migrated cells transfected with EGFP or SLC6A12 are shown. Quantitative analysis of migrated cells was carried out by measuring the absorbance of stained cell extracts at 595 nm (200×, magnification) (A). Migration of serum-starved cells towards 10% serum-containing medium was determined using a Matrigel-coated invasion chamber. Representative images of invading cells transfected with EGFP or SLC6A12 are shown. Quantitative analysis of invading cells was carried out by measuring the absorbance of stained cell extracts at 595 nm (200×, magnification) (B). Data are shown as the mean±SD for triplicate measurements. Statistical analysis was performed using a t-test (^**p<0.01, ^***p<0.001). SLC6A12, solute carrier family 6, member 12.