Table 2.

Vasopressin fiber density distribution in animal facility reared (AFR) and MS rats.

Control				Maternal separation
Bregma (mm)	BLA	CeA	MeA	Bregma (mm)	BLA	CeA	MeA
−1.72	+	++	+++	−1.72	+	++	++++
−1.86	+	++	+++	−1.86	+	++	++++
−2	+	++	+++	−2	+	++	++++
−2.14	−	+	+++	−2.14	+	++	++++
−2.28	+	+	+++	−2.28	+	++	++++
−2.42	+	+	+++	−2.42	+	++	++++
−2.56	−	+	+++	−2.56	++	+	++++
−2.7	−	+	+++	−2.7	++	+	++++
−2.84	+	+	+++	−2.84	++	++	++++
−2.98	+	++	+++	−2.98	++	+	++++

Tracing was performed under camera lucida and calculation of axonal length in a determined region was calculated using ImageJ. Axon length value from Medial Amygdala (MeA) at Bregma level −1.86 mm in maternal separation (MS), was asigned to 100% (++++) = MAX value. For each region, ++++, +++, ++ and + semi-quantitative values were assigned for fiber length values 76–100%, 51–75%, 26–50% and 1–25% of the MAX value.