FIGURE 2.

SA0657 mRNA is overexpressed in a ΔcshB background. Total RNAs were extracted from exponentially growing cells in the following conditions: MH medium at 37°C, MH medium 25°C and in RPMI medium at 37°C, each in biological duplicates. mRNA levels were determined by qRT-PCR and normalized to a stably expressed control gene (hu: SA1305). Expression is shown in arbitrary units, where the WT level at 37°C is set to 1. Unpaired t-test was performed to evaluate statistical significances. ^∗∗ indicates a p-value lower than 0.01.