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. 2016 Nov 25;9:130. doi: 10.3389/fnmol.2016.00130

Figure 5.

Figure 5

The cytoplasmic distribution of full-length APPL changes with the motile behavior of developing neurons. Magnified views of EP cells at progressive stages of embryogenesis, double- immunolabeled with antibodies against nAPPL (green) and cAPPL (magenta) epitopes. (A) Trailing EP cells that had not yet begun to migrate (55–58 HPF) or that had transitioned from migration to axon outgrowth (65 HPF). (B) Leading EP cells that were undergoing active locomotion (55–58 HPF) or that had transitioned from migration to axon outgrowth (65 HPF). Arrowheads indicate examples of the large vesicles labeled with both nAPPL and cAPPL antibodies (white immunolabeling); this vesicle population was markedly more abundant in the non-migratory neurons. (C) Quantification of the number of large vesicles (>100 nm) that apparently contain full-length APPL. At 55 and 58 HPF, the number of large vesicles was significantly reduced in leading EP cells, compared to trailing EP cells (**p = 0.002 and ***p = 0.001, respectively; ns = not significant). At 65 HPF, there was no significant difference in the number of large perinuclear vesicles in trailing vs. leading EP cells (p = 0.106). Statistical comparisons were performed using pairwise Student's two-tailed t-tests; N ≥ 10 per group; histograms show means ± SD. Scale bar in (A,B) = 5 μm.