FIGURE 4.

Effects of compound C and L-NAME on madagascine-exerted vasodilatation. After 40 mM K⁺ induced high perfusion pressure stabilized, Krebs solution containing 40 mM K⁺ + L-NAME (100 μM) and 40 mM K⁺ + compound C (20 μM) was perfused, respectively. And after the perfusion pressure stabilized, Krebs solution containing 40 mM K⁺ + L-NAME (120 μM) + madagascine and 40 mM K⁺ + compound C (20 μM) + madagascine was perfused. Acetylcholine (ACh, 1 nM) treatment is a bolus injection. Vasodilatation response to ACh confirms the intact of endothelium. Data are expressed as the mean ± SD and the response to Krebs solution was set as baseline. Other data are the relative values compared with baseline. ^∗p < 0.05 compared with stable elevated perfusion pressure by Krebs solution containing both 40 mM K⁺ and L-NAME, n = 6 tissues; ^∗∗p < 0.01 compared with stable elevated perfusion pressure by Krebs solution containing 40 mM K⁺ (PSS control), n = 6 tissues.