TABLE 4.
E. coli strains and plasmids used in this study
| Strains and plasmids | Description | Ref. or source |
|---|---|---|
| Strains | ||
| E. coli DH5α | F− ϕ80d lacZΔM15 Δ(lacZYA-argF)U169 recA1 endA1hsdR17 (rk− mk+) supE44 λ−thi-1 gyrA relA1 | 56 |
| E. coli BL21 (DE3) | F− ompT hsdSB (rB−mB−) gal dcm met(DE3) | Novagen |
| Plasmids | ||
| pEC86 | Cmr, product from pEC66 and pACYC184 with E. coli ccmABCDEFGH genes | 40 |
| pET-22b (+) | Apr, T7 promoter, lac operator, C-terminal His tag, pelB leader | Novagen |
| pASK-IBA3 plus | Apr, tetA promoter/operator, C-terminal Strep tag | IBA (Göttingen) |
| pET_ MarpuDRAFT_1194 | Apr, tsdA from M. purpuratum (Marpu_02550) was cloned into pET-22b (+) with NdeI and XhoI, C-terminal His tag | 7 |
| pASK-IBATint_tsdB | Apr; tsdB from T. intermedia cloned into pASK-IBA3 plus with BsaI, C-terminal Strep tag | 1 |
| pASK-IBA3plus-slit1877 | Apr; tsdB from S. lithotrophicus (Slit_1877) cloned into pASK-IBA3 plus with BsaI, C-terminal Strep tag | This study |
| pASK-IBA3plus-slit1878 | Apr; tsdA from S. lithotrophicus (Slit_1878) cloned into pASK-IBA3 plus with BsaI, C-terminal Strep tag | This study |
| pASK-IBA3plus-slit1877-slit1878 | Apr; tsdBA from S. lithotrophica (Slit_1877-Slit_1878) cloned into pASK-IBA3 plus with BsaI, C-terminal Strep tag | This study |
| pET-soxXAK-Strep | Apr, soxXAK, including fragment cloned into pET-22b(+) together with C-terminal Strep tag by use of XbaI and HindIII | This study |
| pET-Alvin2274-C-Strep | Apr, hip gene coding for HiPIP from A. vinosum (Alvin_2274) cloned into pET-soxXAK-Strep with NdeI and NcoI, C-terminal Strep tag | This study |
| pPR-IBAAvtsdA | Apr; tsdA from A. vinosum (Alvin_0091) cloned into pASK-IBA3, C-terminal Strep tag | 1 |
| pASK-IBA3plus_Alvin_2879 | Apr; Alvin_2879 from A. vinosum cloned into pASK-IBA3 plus with BsaI, C-terminal Strep tag | This study |