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. 2016 Sep 29;291(48):24819–24827. doi: 10.1074/jbc.M116.745737

FIGURE 3.

FIGURE 3.

Overexpression of CHCHD4 blocks exercise-induced p53 nuclear activity. A, p21 mRNA and protein levels in skeletal muscle of WT and CHCHD4 Tg mice in unexercised control (−) and 12 h post-exercise (+) states. Tubulin and VDAC served as protein loading controls for whole tissue (total) and the mitochondrial fraction, respectively. n = 4. B, mRNA expression of p53-regulated genes analyzed by RT-PCR array in skeletal muscle of WT and CHCHD4 Tg mice 12 h after a 60-min period of submaximal treadmill exercise. Of the 84 p53 target genes, 26 were induced 2.5-fold or higher only in WT mice after exercise. Color-coded -fold change in gene expression after exercise compared with non-exercised controls is shown (red, induced; green, repressed). n = 3. C, confirmation of some representative exercise-induced p53-regulated genes identified by RT-PCR array. n = 3. Values are mean ± S.E. *, p < 0.05; **, p < 0.01.