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. 2016 Sep 29;291(48):24819–24827. doi: 10.1074/jbc.M116.745737

FIGURE 6.

FIGURE 6.

Knocking down FOXO3 increases CHCHD4 expression. A, CHCHD4 and p21 expression in HCT116 cells with knockdown of CCCP-induced FOXO3 with shRNA. NS, nonspecific shRNA. n = 3. B, CHCHD4 and p21 expression in HCT116 cells with knockdown of serum starvation-induced FOXO3 with shRNA. n = 3. C, p53 immunoblot of nuclear and total cell fractions of HCT116 cells after knockdown of FOXO3 induced by CCCP or serum starvation. D, representative confocal immunofluorescence image of p53 (red) in HCT116 cells after knockdown of FOXO3 induced by CCCP or serum starvation. DAPI stain (blue) shows nuclei. Values are mean ± S.E. *, p < 0.05; **, p < 0.01; ***, p < 0.001. Scale bar = 10 μm.