FIGURE 7.

Palmitoylation-deficient Dsg2/GFP CACS is degraded more rapidly than wild-type Dsg2/GFP. A, A431 cells expressing wild-type Dsg2/GFP or Dsg2/GFP CACS were grown in medium containing 30 μg/ml cycloheximide (CHX) for the indicated times. Cell lysates were prepared, and equal protein was loaded for immunoblot analysis (cycloheximide treatments were performed in triplicate, and representative blots are shown). B, band intensities were collected using Odyssey infrared scanning of three independent experiments. Error bars represent the standard deviation. C and D, subcellular localization of Dsg2/GFP CACS stably expressed in A431 cells that were grown in control medium and in medium containing 30 μg/ml cycloheximide for 3 h.