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. 2016 Oct 7;291(48):24892–24899. doi: 10.1074/jbc.M116.734673

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© 2016 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 1. — CBFβ interferes with the interaction between Vif and MDM2. A, co-immunoprecipitation of MDM2 with Vif wild-type and variants that do not interact with CBFβ. 293T cells transiently expressing myc-tagged Vif wild-type or the variant and HA-tagged MDM2 were lysed and immunoprecipitated by anti-myc serum, and samples were analyzed by immunoblotting (IB) with anti-HA, anti-myc, and anti-CBFβ sera. B, reciprocal co-immunoprecipitation of Vif wild-type and variants with MDM2. Lysates of 293T cells with overexpression of untagged Vif wild-type or the variant and myc-tagged MDM2 were immunoprecipitated by anti-myc serum, and samples were analyzed by immunoblotting with anti-Vif and anti-myc sera. C, co-immunoprecipitation of MDM2 with Vif wild-type in the presence of CBFβ overexpression. Lysates of 293T cells transiently expressing myc-tagged Vif, HA-tagged MDM2, and various amounts of CBFβ were immunoprecipitated by anti-myc serum and analyzed for co-precipitation of MDM2 by immunoblotting.