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ZmSSIIa activity quantified by ADP release using the ADP-Glo reagent (Promega). A, ZmSSIIa activity using maltohexaose (DP6) or glycogen as acceptor molecules. B, the ADP-glucose preparation contributed to observed signal in the assay that was less than the ADP generated in reactions containing the combination of ADP-glucose, an acceptor substrate and SSIIa.
